Department of Biochemical Engineering and Biotechnology
About My Lab
My lab is currently working in the following three areas.
laccases of Cyathus bulleri in degradation of textile dyes and textile effluents.
laccases of Cyathus bulleri in degradation of textile dyes and textile effluents. Molecular and Biochemical tools are used to investigate the role of laccase isoforms in various bioremediation methods. Engineering of laccases to generate hyper-catalytic variants of laccases, with improved biochemical properties, have been generated using protein engineering and metal replacement strategies (Insert Picture
a) Superimposed modelled structures of laccase isoforms (Lcc1: Green, Lcc2: Yellow, Lcc3: Pink, Lcc4: Orange, Lcc5: Blue, Lcc6: Grey); Spheres in red represent four copper atoms. White arrows indicate dissimilar regions in the structure.
b) Active sites of laccase isoforms (Lcc1-6) with dissimilar amino acid residues at equivalent positions. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of J. Hazard. Material. (2018) 344, 466)
Production of human therapeutics in Pichia pastoris expression system.
Production of human therapeutics in Pichia pastoris expression system. Basic molecular biology and medium optimization strategies have been applied to increase production of Human Serum Albumin and Granulocyte Colony-Stimulating Factor. Transcriptome analyses has been carried out to understand factors at molecular level that regulate production of foreign proteins. Presented below is a net work of activities identified to be up- or down-regulated during production of Human Serum Albumin in P. pastoris.
a) Genes regulated in carbon metabolism under optimized conditions b) Genes regulated in nitrogen metabolism according to KEGG pathways under optimized and unoptimized conditions. Intensity of up- (shown in red) and down-regulated (green) genes is shown by the number of arrows. One arrow represents log2fold unit change of 1 (For details, see Biomolecules (2019) 9, 568)
Cell catalyzed biosynthesis of Fructo-oligosaccharides. Both free and immobilized bacterial cells are used to drive synthesis of specific oligosaccharides.
B. A. (Biology Major, Maths Minor, 1st three semesters) University of Pennsylvania, Philadelphia, PA USA, 1970-71.
B. Sc. (Botany, Zoology, Chemistry, Higher English) University of Delhi, India. 1st rank in group B, 1975.
M. A. (Biology) City University of New-York, N.Y., USA, 1980.
Ph. D. (Molecular Genetics) City University of New-York, N.Y. USA, 1980.
Post-Doc: (Molecular Biology) Department of Biotechnology, Institute Pasteur, Paris,
France (May 1989 – May 1990)
(Fungal Molecular Biology) VTT Biotechnical Laboratory, Espoo, Finland (May
(Molecular Biology) University of California, Davis, USA (Jan 1993-July 1993).